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The majority of the begomoviruses are bipartite and the genomic components are referred as DNA-A and DNA-B.
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Begomoviruses are transmitted by whitefly Bemisia tabaci (Gennadius) predominantly in the tropical and subtropical regions of the world and cause severe disease in dicot plants including tomato, pepper, cassava, beans, cotton and cucurbits. A large number of important diseases are caused by begomoviruses, which comprises of more than 180 species. They are classified into four genera: Mastrevirus, Curtovirus, Topocuvirus and Begomovirus, on the basis of their genome organization, host range and insect vector. They infect a wide range of monocotyledonous and dicotyledonous plants but each member of the Geminiviridae family has its own limited host range. Geminiviruses (family Geminiviridae) possess monopartite or bipartite circular, single-stranded DNA genomes encapsidated in geminate (18-22 nm diameters) particles. The level of viral DNA accumulation in the diseased plants Infectivity of the cloned genomic componentsģ.4. Field detection of the viral disease and cloning of viral DNAģ.3. Analysis of viral DNA in inoculated plantsģ.1. Construction of agroinfectious clones and their infectivityĢ.6. MOLECULAR CHARACTERIZATION AND INFECTIVITY OF A TOMATO LEAF CURL NEW DELHI VIRUS ASSOCIATED WITH NEWLY EMERGING YELLOW MOSAIC DISEASE OF EGGPLANT IN INDIAĢ.5.